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The Triglyceride colorimetric assay kit is specifically designed for in vitro use to accurately measure the concentration of triglycerides in human serum. This assay provides a quantitative determination of triglyceride levels and is suitable for various research and clinical applications.
Triglycerides can be found in various tissues throughout the human body, but a majority of them, around 89%, are stored in adipose tissue. There are many factors that can impact the amount of triglycerides in the bloodstream, with conditions such as atherosclerosis, diabetes, nephrotic syndrome, and primary or secondary hyperlipoproteinemia causing an increase in serum triglyceride levels. In contrast, conditions like primary lipoprotein deficiency, hyperthyroidism, adrenal insufficiency, and poor digestion and absorption can lead to lower levels of triglycerides in the serum.
Use Procedures for Triglyceride colorimetric assay kit：
REAGENTS |
Blank (B) |
Standard (S) |
Test (T) |
Reagent 1 （μL） |
1000 |
1000 |
1000 |
Distilled Water（μL） |
10uL |
_ |
_ |
Standard（μL） |
_ |
10 |
_ |
Sample（μL） |
_ |
_ |
10 |
After carefully mixing the components, place them in an incubator at a temperature of 37 ℃ for a duration of 10 minutes. Once the incubation period is complete, proceed to measure the absorbance levels of both the test (T) and the standard (S) samples relative to the Reagent Blank at a wavelength of 500 nm. | |||
Specification:
500ml/1L/5L/10L/20L/50L.
Storage condition:
The Triglyceride colorimetric assay kit should be stored in a cool, dark place at a temperature between 2 ℃ and 8 ℃. Its shelf life is 18 months from the date of manufacture. If you have already opened the kit, make sure to store it in the same conditions as instructed. The opened kit can last up to 28 days under proper storage conditions. It's essential to follow these guidelines to ensure accurate and reliable results when using this kit.
Data sheet:
Detecction Method |
GPO-PAP Method and End Point Method |
Wave |
500nm |
Sample |
Fresh serum without hemolysis. |
Assay type |
Quantitative |
Linearity |
For CHO up to 11.4 mmol/L. |
Accuracy |
To ensure the accuracy of reference materials, the relative deviation must be maintained within the range of ± 15%. This is a crucial factor in determining the reliability of the reference materials. Any deviation beyond this limit can significantly affect the results obtained from the materials, making them unreliable and inaccurate. Therefore, it is essential to maintain strict adherence to the prescribed deviation range to ensure the validity of the reference materials used in any analysis or experiment. |
Precision |
The run repeatability coefficient of variation (CV) should be no more than 5%, while the relative range between runs should be maintained. To achieve this, it is important to rearrange and organize the content while preserving the original information. |
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